######################################## # Program: restrict # Rundate: Tue May 30 14:35:16 2006 # Report_format: table # Report_file: phat4_unique.restrict ######################################## #======================================= # # Sequence: phat4.seq from: 1 to: 4527 # HitCount: 45 # # Minimum cuts per enzyme: 1 # Maximum cuts per enzyme: 1 # Minimum length of recognition site: 4 # Blunt ends allowed # Sticky ends allowed # DNA is circular # Ambiguities allowed # #======================================= Start End Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 119 124 SpeI ACTAGT 119 123 . . 195 200 DsaI CCRYGG 195 199 . . 195 200 NcoI CCATGG 195 199 . . 206 211 EcoRI GAATTC 206 210 . . 212 217 SalI GTCGAC 212 216 . . 212 217 AccI GTMKAC 213 215 . . 218 223 XhoI CTCGAG 218 222 . . 218 223 AvaI CYCGRG 218 222 . . 251 256 EcoT22I ATGCAT 255 251 . . 251 256 BfrBI ATGCAT 253 253 . . 257 262 HindIII AAGCTT 257 261 . . 347 353 EspI GCTNAGC 348 351 . . 425 430 BspMII TCCGGA 425 429 . . 599 604 PsiI TTATAA 601 601 . . 671 683 AloI GAACNNNNNNTCC 663 658 695 690 740 747 Sse8387I CCTGCAGG 745 741 . . 741 746 PstI CTGCAG 745 741 . . 748 753 BspMI ACCTGC 734 738 . . 904 909 NarI GGCGCC 905 907 . . 904 909 KasI GGCGCC 904 908 . . 904 909 EgeI GGCGCC 906 906 . . 904 909 BbeI GGCGCC 908 904 . . 1132 1137 BsePI GCGCGC 1132 1136 . . 1361 1367 BstEII GGTNACC 1361 1366 . . 1529 1534 BclI TGATCA 1529 1533 . . 1543 1548 MluI ACGCGT 1543 1547 . . 1962 1972 PflMI CCANNNNNTGG 1968 1965 . . 1978 1983 KpnI GGTACC 1982 1978 . . 1978 1983 Acc65I GGTACC 1978 1982 . . 1984 1989 SacI GAGCTC 1988 1984 . . 1984 1989 Ecl136II GAGCTC 1986 1986 . . 1995 2000 BsaAI YACGTR 1997 1997 . . 2098 2103 NaeI GCCGGC 2100 2100 . . 2098 2103 MroNI GCCGGC 2098 2102 . . 2279 2284 NdeI CATATG 2280 2282 . . 2415 2421 PfoI TCCNGGA 2415 2420 . . 2531 2536 ZraI GACGTC 2533 2533 . . 2531 2536 AatII GACGTC 2535 2531 . . 2850 2859 XmnI GAANNNNTTC 2854 2854 . . 2971 2976 ScaI AGTACT 2973 2973 . . 3382 3387 Eco31I GGTCTC 3388 3392 . . 3449 3459 Eam1105I GACNNNNNGTC 3454 3453 . . 3928 3936 AlwNI CAGNNNCTG 3933 3930 . . 4342 4347 BspLU11I ACATGT 4342 4346 . . 4470 4476 SapI GCTCTTC 4459 4462 . . #--------------------------------------- #---------------------------------------