Month: May 2022

Divergent binding mode for a protozoan BRC repeat to RAD51

Teodors Pantelejevs and Marko Hyvönen

Biochemical Journal, 479: 1031–1043 (2022)
DOI: 10.1042/BCJ20220141
Pubmed: 35502837
BioRxiv preprint: 2022.01.25.477309v1

PDB coordinates:
7QV8 (3D view)
L.infantum RAD51 complexed with BRC repeat


Interaction of BRCA2 through ca. 30 amino acid residue motifs, BRC repeats, with RAD51 is a conserved feature of the double-strand DNA break repair by homologous recombination in eukaryotes. In humans the binding of the eight BRC repeats is defined by two sequence motifs, FxxA and LFDE, interacting with distinct sites on RAD51. Little is known of the interaction of BRC repeats in other species, especially in protozoans, where variable number of BRC repeats are found in BRCA2 proteins. Here we have studied in detail the interactions of the two BRC repeats in Leishmania infantum BRCA2 with RAD51. We show LiBRC1 is a high-affinity repeat and determine the crystal structure of its complex with LiRAD51. Continue reading →

Posted by Marko in Publications, 0 comments