Here are links to some of the methods used and resources avaliable in our group.
Feel free to use. If useful, thanks are always gratefully accepted!
- Cloning an expression construct (aka “The vintage manual of cloning”)
- A more modern version for SLIC cloning
- Testing for expression and solubility
- Large scale expression with pBAT vectors
- Protein quantification with UV absorbance (updated 2017)
- SDS-PAGE
- Zinc-Imidazole staining for SDS-PAGE gels
- Inclusion body preparation
- Western blotting
You might also find useful the following cloning, expression etc. related materials:
- E. coli expression vectors
- pBAT series of T7 expression vectors we have used for years
- The improved version of these vectors, our new pOP series of plasmids
- Vectors with a wider range of fusions and for SLIC: the pExp series of vectors
Some slides from methods talks
- Slides from 2022 postgraduate course on protein production at University of São Paulo