Month: February 2023

Going 3D, really 3D

After 20+ years of determining atomic level structures of proteins in three dimensions, we are going properly 3D. Our Prusa i3 MKS3+ has arrived!

Posted by Marko in News

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture

Philipp KnyphausenMariana Rangel PereiraPaul Brear, Marko HyvönenLutz JermutusFlorian Hollfelder

Nature Communications 14:768 (2023)
DOI: 10.1038/s41467-023-36099-7
Pubmed: 36765057
PDB coordinates:6YV5 (3D view ), 6YV6 (3D view )


Tailoring of the activity and specificity of proteases is critical for their utility across industrial, medical and research purposes. However, engineering or evolving protease catalysts is challenging and often labour intensive. Here, we describe a generic method to accelerate this process based on yeast display. We introduce the protease selection system A2Mcap that covalently captures protease catalysts by repurposed alpha-2-macroglobulin (A2Ms). To demonstrate the utility of A2Mcap for protease engineering we exemplify the directed activity and specificity evolution of six serine proteases. This resulted in a variant of Staphylococcus aureus serin-protease-like (Spl) protease SplB, an enzyme used for recombinant protein processing, that no longer requires activation by N-terminal signal peptide removal. Continue reading →

Posted by Marko in Publications, 0 comments

One of those weeks

HPLC plays up with random UV peaks, fraction collector tries to fail its calibration, centrifuge bottles crack with half of mammalian expression culture lost, fluorescent detector cuts off randomly.

And it’s only a Wednesday.

Panton Arms, here we come.

Posted by Marko in News