Manuela Urbischek, Helena Rannikmae, Thomas Foets, Katharina Ravn, Marko Hyvönen & Marc de la Roche
Scientific Reports 6193 (2019)
DOI: 0.1038/s41598-019-42604-0
Pubmed: 30996238
Abstract
The media formulations necessary for deriving and sustaining organoids from epithelial tissues such as prostate, colon, gastric, liver, pancreas, and others have been established. Critical components of organoid media are a set of growth factors that include R-spondins and BMP signalling antagonists such as Noggin or Gremlin 1. Currently, the practical limitations for formulating organoid media of reproducible potency and larger-scale media production that have hampered further technological applications of organoid technology include: the cost of growth factors such as R-spondins and Gremlin 1/Noggin and their production as defined specific activities free of contaminants that may affect organoid growth. Continue reading →